Taw qhia rau BSA Protein
Bovine serum albumin (BSA) yog globular protein ib txwm muaj nyob rau hauv bovine ntshav plasma thiab feem ntau zoo zoo los ntawm bovine serum raws li ib tug byproduct ntawm nyuj kev lag luam. Vim tias nws ruaj khov, pheej yig, thiab ua haujlwm tau zoo, BSA tau dhau los ua tus qauv reagent hauv biochemical, molecular biology, kuaj mob, thiab siv bioprocessing.
bovine serum albumin yog dab tsi?
BSA yog ib qho globular, uas tsis yog-glycosylated-helical plasma protein tsim nyob rau hauv daim siab bovine. Nws suav nrog ib qho kev sib txuas ntawm polypeptide ntawm kwv yees li 583 amino acid residues thiab muaj 17 intrachain disulfide txuas hniav, uas ua rau muaj kev ruaj ntseg tshwj xeeb. BSA nthuav tawm cov kev hloov pauv hloov pauv ntawm isomerization uas nyob ntawm pH thiab qhov kub thiab txias, ua kom nws khi thiab thauj ntau yam endogenous thiab exogenous ligands, suav nrog fatty acids, amino acids, steroids, hlau ions, thiab tshuaj tshuaj.
Lyophilized daim ntawv
Cov lyophilized (freeze-dried) hmoov hom muaj kev ruaj ntseg tshwj xeeb, lub neej ntev txee, yooj yim tuav, thiab yooj yim ntawm kev thauj mus los piv rau cov kua formulations. Lyophilization khaws cia cov dej num lom neeg thaum ua kom muaj qhov hnyav thiab rov tsim dua ntawm qhov xav tau ntau yam rau ntau yam kev tshawb fawb thiab kev siv tshuab. Hom ntawv no txhim kho kev tuav, thauj, thiab khaws cia yooj yim, ua rau nws tsim nyog rau kev faib thoob ntiaj teb thiab kev tswj cov khoom muag mus ntev.
Certificate of Analysis (COA)
| Test Parameter | Specification | Yam tshwm sim | Txoj kev |
|---|---|---|---|
| Qhov tshwm sim | Tawm -dawb mus rau daj ntseg daj lyophilized hmoov | Ua raws | Kev tshuaj xyuas qhov muag |
| Tus Kheej (PAGE / Immunoassay) | Zoo rau bovine serum albumin | Qhov zoo | SDS-PAGE / Immunodiffusion |
| Purity (Agarose Gel Electrophoresis) | Ntau dua lossis sib npaug li 96% - Ntau dua lossis sib npaug rau 99% (qib nyob ntawm) | Ntau dua lossis sib npaug li 98.5% | Agarose Gel Electrophoresis / HPLC |
| Molecular Luj (SDS-PAGE) | ~ 66 kDa (monomer) | 65,000 – 67,000 Da | SDS-PAGE / SEC-MALS |
| pH (1% tov hauv dej) | 6.5 – 7.5 | 7.1 | pH Meter |
| Poob rau ziab | Tsawg dua lossis sib npaug li 5.0% (w / w) | Tsawg dua lossis sib npaug li 2.0% - Tsawg dua lossis sib npaug rau 5.0% | Gravimetric / TGA |
| Endotoxin (LAL xeem) | <1.0 – <5.0 EU/mg (grade dependent); low endotoxin: <0.1 EU/mg | <0.5 EU/mg | LAL (USP<85>) |
| Protease Activity | Tsis muaj qhov kuaj pom lossis Tsawg dua lossis sib npaug li 0.005 U / mg | Tsis pom muaj leej twg | Fluorescence / Casein Digestion |
| Fatty Acid Cov ntsiab lus | Tsawg dua lossis sib npaug li 0.02% - Tsawg dua lossis sib npaug rau 0.05% (FA-dawb: Tsawg dua lossis sib npaug rau 0.02%; tus qauv: Tsawg dua lossis sib npaug rau 0.05%) | Tsawg dua lossis sib npaug li 0.02% | Enzymatic / GC |
| Bovine IgG | Hauv qab no txwv tsis pub muaj (<1.0 μg/mg) | <0.5 μg/mg | ELISA |
| Microbial Bioburden | <3 CFU/mL (typically no growth) | <1 CFU/mL | Phaj suav (ISO 4833) |
| Fungal suav | <1 CFU/mL | 0 CFU/ml | Sabouraud Agar |
| Kev kuaj kab mob | Tsis zoo rau BVDV, IBR, BTV, VSV, Reovirus 3, Parainfluenza 3 | Tsis zoo | qPCR / Cell Culture |
| Solubility (40 mg / ml hauv dej) | Ntshiab rau me ntsis hazy, tsis muaj particulates | Clear daws | Visual / Turbidity |
| Heavy Metals (Pb, As, Cd, Hg) | Tsawg dua lossis sib npaug li 10 ppm txhua | <1 ppm | ICP-MS |
| Kev cia khoom | 2-8 degree, desiccated, kaw | N/A | N/A |
| Lub neej txee (tsis qhib) | 5 xyoo txij li hnub tsim khoom | N/A | Kev kawm ruaj khov |
Nco tseg: Muaj COA cov ntsiab lus nrog ntau- cov txiaj ntsig kev xeem tshwj xeeb yog muab rau txhua qhov khoom xa tuaj. Cov kev ntsuas ntxiv (piv txwv li, DNase/RNase kev ua haujlwm, molecular biology- qib specifications, qis-endotoxin specifications, fatty acid- ntawv pov thawj pub dawb) muaj nyob rau ntawm kev thov.
Kev tsim khoom
Kev tsim khoom ntawm siab -purity BSA lyophilized hmoov ua raws li cov txheej txheem tswj hwm, ua kom cov txheej txheem kom ntseeg tau batch-rau-batch sib xws thiab kev nyab xeeb:
- Plasma Collection- Cov ntshav hauv plab yog sau los ntawm kev noj qab haus huv, tsoomfwv -kuaj nyuj nyob rau hauv kev saib xyuas ntawm kws kho tsiaj. Plasma yog cais los ntawm cov cellular Cheebtsam.
- Serum Processing- Plasma yog clotted thiab lim kom tshem tawm fibrin, yielding clarified bovine serum.
- Purification (Cold Ethanol Fractionation - Hloov Cohn Method)- Serum albumin cais raws li "Fraction V" siv tswj pH, ethanol concentration, qis kub (-5℃rau 0 degree), thiab ionic zog.
- Diafiltration & Buffer Exchange- Tshem tawm cov seem ethanol thiab ntsev thaum kho pH thiab conductivity.
- Sterile Filtration- 0.22 μm pom kom tsis muaj menyuam.
- Lyophilization- Cov tshuaj BSA tsis muaj menyuam yog khov-qhuav kom ua tiav cov dej seem seem tsawg dua lossis sib npaug li 5%, ua cov hmoov ruaj khov, tsis muaj dej ntws.
- QC kuaj & tso tawm- Txhua pawg tau txais kev sim ua tiav (purity, tus kheej, endotoxin, bioburden, kab mob kev nyab xeeb, thiab lwm yam) raws li COA.
- Ntim & Cia- Ntim rau hauv cov thawv uas tsis muaj menyuam, tsis tshua muaj me me nyob rau hauv kev tswj cov av noo. Khaws ntawm 2-8 degree.
bovine serum albumin siv rau dab tsi?
Cell Culture
BSA muab tshuaj dawb dawb los yog txo cov ntshav qab zib. Nws txhawb nqa cell proliferation thiab viability los ntawm stabilizing kev loj hlob yam, khi toxic fatty acids, thiab txo oxidative kev nyuaj siab.
Protein Quantitation Standard
BSA yog thawj tus qauv siv rau Bradford, BCA, Lowry, thiab biuret assay. USP-qib BSA xyuas kom meej, ntsuas cov protein ntau dua.
Immunoassay Blocker & Diluent
BSA saturates cov chaw tsis tshwj xeeb khi hauv ELISA, Western blot, thiab IHC, txo cov keeb kwm yav dhau. Nws kuj stabilizes dilute cov tshuaj tiv thaiv kab mob ua cov kab mob protein.
Enzyme Stabilization
BSA tiv thaiv denaturation, deg adsorption, thiab proteolytic degradation ntawm purified enzymes thaum khaws cia thiab kev sim.
Kev xa tshuaj & Nanocarrier Systems
BSA cov ntaub ntawv biocompatible, biodegradable nanoparticles rau lub hom phiaj xa tshuaj. Nws txhim kho cov solubility ntawm cov tshuaj hydrophobic, muaj kev tswj kev tso tawm, thiab txhim kho bioavailability.
Cov lus nug nquag nug (FAQ)
Q: Cov khoom no puas haum rau kev kuaj mob lossis kev siv kho mob?
A: Tsis yog. Nws yuav tsum tau muab tso rau hauv kev tshawb fawb lossis kev lag luam reagent nkaus xwb, tsis yog khoom kho mob lossis chaw kho mob.
Q: Cov khoom no puas haum rau kev kuaj mob lossis kev siv kho mob?
A: Tsis yog. Nws yuav tsum tau muab tso rau hauv kev tshawb fawb lossis kev lag luam reagent nkaus xwb, tsis yog khoom kho mob lossis chaw kho mob.
Q: Cov qib twg feem ntau muaj?
A: Cov kev xaiv muaj xws li qib txheem, qib crystallized, fatty-acid- qib dawb, protease- qib dawb, thiab qis- qib endotoxin, nyob ntawm cov neeg siv khoom xav tau.
Q: BSA puas tuaj yeem siv rau hauv ELISA thaiv cov buffers?
A: Yog. BSA yog ib qho ntawm cov tshuaj tiv thaiv ntau tshaj plaws hauv ELISA vim nws pab txo qis adsorption.
Q: Koj puas muab Daim Ntawv Pov Thawj Kev Ntsuam Xyuas (COA) rau txhua pawg?
A: Yog lawm, txhua qhov khoom xa tuaj nrog ntau ntau - Daim Ntawv Pov Thawj Tshwj Xeeb ntawm Kev Ntsuas.
Q: Puas yog BSA haum rau siv hauv cov kab lis kev cai tsis muaj ntshav qab zib?
A: Yog lawm, BSA yog ib qho tseem ceeb hauv ntau cov tshuaj uas tsis muaj ntshav qab zib. Nws ua haujlwm raws li cov neeg nqa khoom noj protein rau lipids, cov tshuaj hormones, thiab kev loj hlob, thaum tseem tiv thaiv kev ntxhov siab thiab kev puas tsuaj oxidative. Fatty acid-free BSA raug pom zoo thaum muaj kev tswj xyuas meej ntawm fatty acid muaj pes tsawg leeg.
Q: Puas yog BSA haum rau hauv vivo tsiaj tshawb fawb thiab tshuaj tshuaj?
A: BSA npaj rau hauv vivo thiab kev siv tshuaj yuav tsum ua kom tau raws li cov lus qhia nruj, suav nrog cov tshuaj endotoxin qis (<0.1 EU/mg), sterility, and viral clearance validation. We offer BSA grades specifically designed for such applications. Please consult with our technical support team to select the appropriate grade and provide relevant regulatory documentation.
Vim li cas thiaj xaiv peb?
Koj tus kws tshaj lij kev tsim khoom & Sourcing tus khub
Peb yog ntau tshaj li tus neeg muag khoom - peb yog cov khoom lag luam sib txuas ua ke rau siab - cov khoom xyaw zoo, cov qauv kev cai, thiab cov khoom tiav thoob plaws ntau qhov kev lag luam.
Cov kws tshaj lij
Nrog rau ntau xyoo ntawm kev paub hauv cov nroj tsuag extracts, zaub mov additives, cosmetic raw cov ntaub ntawv, thiab cov khoom noj khoom haus, peb pab neeg coj kev txawj ntse txawj ntse thiab kev ua lag luam kev pom zoo rau txhua qhov kev koom tes.
01
Ntau Yam Khoom Muag
Peb muab ntau yam khoom lag luam, suav nrog cov khoom noj khoom haus ntxiv, cov khoom siv cog qoob loo, txiv hmab txiv ntoo & zaub hmoov, tshuaj pleev ib ce, thiab cov khoom xyaw ua zaub mov.
02
Ib -Stop Shop
Raw Khoom:High -cov khoom xyaw purity rau zaub mov, dej haus, ntxiv, thiab kev siv tshuaj pleev ib ce.
Cov khoom tiav:Hauv -tsev tsim cov tshuaj ntsiav, mos gels, gummies, kua tee, thiab hmoov sib tov.
03
Kev pabcuam OEM / ODM / OBM
Label Design & Ntim:Custom branding tailored rau koj lub hom phiaj kev lag luam.
Formulation & R & D:Cov kws tshaj lij kev txhawb nqa los tsim cov kev cai sib xyaw, cov qauv tsim muaj, thiab cov khoom siv tshiab.
Ntiaj teb no Logistics:Txhim khu kev xa khoom, cov ntaub ntawv, thiab kev pabcuam kev lis kev cai.
04
Quality Assurance
nruj me ntsis ua raws li GMP cov qauv thiab tag nrho cov kev cai pob tshab. Peb nyob twj ywm tam sim no nrog cov kev cai thoob ntiaj teb, suav nrog FDA GRAS, EFSA, thiab lwm yam kev cai hauv cheeb tsam, kom ntseeg tau tias koj cov khoom ua tau raws li cov qauv kev nyab xeeb siab tshaj plaws thiab ua raws.
05
Tiv tauj peb
Hu rau peb pab neeg muag khoom los tham txog koj cov kev xav tau rauBovine Serum Albumin Lyophilized Hmoov.
Thov hu rau peb ntawmella.zhang@huilinbio-tech.com.
Tsis lees paub
Cov ntaub ntawv no yog tsim los rau cov ntaub ntawv xov xwm thiab kev tshawb fawb nkaus xwb. BSA yog muab rau kev tshawb fawb hauv chaw soj nstuam, kuaj hauv vitro, bioprocessing, thiab kev siv tshuaj siv tshuaj raws li muaj. Tsis yog siv rau tib neeg lossis tsiaj kho mob tshwj tsis yog lwm yam tau teev tseg thiab pom zoo rau kev siv. Cov neeg siv yog lub luag haujlwm los xyuas kom meej ua raws li tag nrho cov kev cai hauv zos, lub teb chaws, thiab thoob ntiaj teb kev tswj hwm kev siv tsiaj- cov khoom tau los.
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